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Development and Validation of an Affinity Chromatography-Protein G Method for IgG Quantification

Nimotuzumab, an IgG that recognizes the epidermal growth factor receptor (EGF-R) overexpressed in some tumors, is used in the treatment of advanced head and neck cancer. For the quantification of this protein in cell culture supernatants, protein G-HPLC affinity chromatography is used due to its hig...

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Detalles Bibliográficos
Autores principales: Paradina Fernández, Lesly, Calvo, Loany, Viña, Lisel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4897240/
https://www.ncbi.nlm.nih.gov/pubmed/27379284
http://dx.doi.org/10.1155/2014/487101