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Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules

PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes. However, ‘universal’ SSU rRNA gene PCR primer sets are likely to miss much of the diversity therein. We sequ...

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Detalles Bibliográficos
Autores principales: McDonald, James E., Larsen, Niels, Pennington, Andrea, Connolly, John, Wallis, Corrin, Rooks, David J., Hall, Neil, McCarthy, Alan J., Allison, Heather E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4898712/
https://www.ncbi.nlm.nih.gov/pubmed/27276347
http://dx.doi.org/10.1371/journal.pone.0157046