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Characterising the Canine Oral Microbiome by Direct Sequencing of Reverse-Transcribed rRNA Molecules
PCR amplification and sequencing of phylogenetic markers, primarily Small Sub-Unit ribosomal RNA (SSU rRNA) genes, has been the paradigm for defining the taxonomic composition of microbiomes. However, ‘universal’ SSU rRNA gene PCR primer sets are likely to miss much of the diversity therein. We sequ...
Autores principales: | McDonald, James E., Larsen, Niels, Pennington, Andrea, Connolly, John, Wallis, Corrin, Rooks, David J., Hall, Neil, McCarthy, Alan J., Allison, Heather E. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4898712/ https://www.ncbi.nlm.nih.gov/pubmed/27276347 http://dx.doi.org/10.1371/journal.pone.0157046 |
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