Targeted, homology-driven gene insertion in stem cells by ZFN-loaded ‘all-in-one’ lentiviral vectors

Biased integration remains a key challenge for gene therapy based on lentiviral vector technologies. Engineering of next-generation lentiviral vectors targeting safe genomic harbors for insertion is therefore of high relevance. In a previous paper (Cai et al., 2014a), we showed the use of integrase-...

Descripción completa

Detalles Bibliográficos
Autores principales: Cai, Yujia, Laustsen, Anders, Zhou, Yan, Sun, Chenglong, Anderson, Mads Valdemar, Li, Shengting, Uldbjerg, Niels, Luo, Yonglun, Jakobsen, Martin R, Mikkelsen, Jacob Giehm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2016
Materias:
Developmental Biology and Stem Cells
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900802/
https://www.ncbi.nlm.nih.gov/pubmed/27278774
http://dx.doi.org/10.7554/eLife.12213

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900802/
https://www.ncbi.nlm.nih.gov/pubmed/27278774
http://dx.doi.org/10.7554/eLife.12213

Ejemplares similares