Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes

We compare the ability of shRNA and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We find that the precision of the two libraries in detecting essential genes is similar and that combining data from both screens improves performance. Notabl...

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Detalles Bibliográficos
Autores principales: Morgens, David W, Deans, Richard M, Li, Amy, Bassik, Michael C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900911/
https://www.ncbi.nlm.nih.gov/pubmed/27159373
http://dx.doi.org/10.1038/nbt.3567

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900911/
https://www.ncbi.nlm.nih.gov/pubmed/27159373
http://dx.doi.org/10.1038/nbt.3567

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