Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes

We compare the ability of shRNA and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We find that the precision of the two libraries in detecting essential genes is similar and that combining data from both screens improves performance. Notabl...

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Detalles Bibliográficos
Autores principales: Morgens, David W, Deans, Richard M, Li, Amy, Bassik, Michael C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900911/
https://www.ncbi.nlm.nih.gov/pubmed/27159373
http://dx.doi.org/10.1038/nbt.3567
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author Morgens, David W
Deans, Richard M
Li, Amy
Bassik, Michael C
author_facet Morgens, David W
Deans, Richard M
Li, Amy
Bassik, Michael C
author_sort Morgens, David W
collection PubMed
description We compare the ability of shRNA and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We find that the precision of the two libraries in detecting essential genes is similar and that combining data from both screens improves performance. Notably, results from the two screens show little correlation, which can be partially explained by identification of distinct essential biological processes with each technology.
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spelling pubmed-49009112016-11-09 Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes Morgens, David W Deans, Richard M Li, Amy Bassik, Michael C Nat Biotechnol Article We compare the ability of shRNA and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We find that the precision of the two libraries in detecting essential genes is similar and that combining data from both screens improves performance. Notably, results from the two screens show little correlation, which can be partially explained by identification of distinct essential biological processes with each technology. 2016-05-09 2016-06 /pmc/articles/PMC4900911/ /pubmed/27159373 http://dx.doi.org/10.1038/nbt.3567 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Morgens, David W
Deans, Richard M
Li, Amy
Bassik, Michael C
Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes
title Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes
title_full Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes
title_fullStr Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes
title_full_unstemmed Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes
title_short Systematic comparison of CRISPR-Cas9 and RNAi screens for essential genes
title_sort systematic comparison of crispr-cas9 and rnai screens for essential genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900911/
https://www.ncbi.nlm.nih.gov/pubmed/27159373
http://dx.doi.org/10.1038/nbt.3567
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