Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch

The CRISPR-associated protein Cas9 from Streptococcus pyogenes is an RNA-guided DNA endonuclease with widespread utility for genome modification. However, the structural constraints limiting the engineering of Cas9 have not been determined. Here we experimentally profile Cas9 using randomized insert...

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Detalles Bibliográficos
Autores principales: Oakes, Benjamin L, Nadler, Dana C., Flamholz, Avi, Fellmann, Christof, Staahl, Brett T., Doudna, Jennifer A., Savage, David F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900928/
https://www.ncbi.nlm.nih.gov/pubmed/27136077
http://dx.doi.org/10.1038/nbt.3528

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900928/
https://www.ncbi.nlm.nih.gov/pubmed/27136077
http://dx.doi.org/10.1038/nbt.3528

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