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Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch
The CRISPR-associated protein Cas9 from Streptococcus pyogenes is an RNA-guided DNA endonuclease with widespread utility for genome modification. However, the structural constraints limiting the engineering of Cas9 have not been determined. Here we experimentally profile Cas9 using randomized insert...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4900928/ https://www.ncbi.nlm.nih.gov/pubmed/27136077 http://dx.doi.org/10.1038/nbt.3528 |