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Improving analytical methods for protein-protein interaction through implementation of chemically inducible dimerization

When investigating interactions between two proteins with complementary reporter tags in yeast two-hybrid or split GFP assays, it remains troublesome to discriminate true- from false-negative results and challenging to compare the level of interaction across experiments. This leads to decreased sens...

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Detalles Bibliográficos
Autores principales: Andersen, Tonni Grube, Nintemann, Sebastian J., Marek, Magdalena, Halkier, Barbara A., Schulz, Alexander, Burow, Meike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4901268/
https://www.ncbi.nlm.nih.gov/pubmed/27282591
http://dx.doi.org/10.1038/srep27766