Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling

It is suggested that oxLDL is decisive in the initiation and development of atherosclerotic injuries. The up-regulation of oxidative stress and the generation of ROS act as key modulators in developing pro-atherosclerotic and anti-atherosclerotic processes in the human endothelial wall. In this pres...

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Autores principales: Hung, Ching-Hsia, Chan, Shih-Hung, Chu, Pei-Ming, Lin, Huei-Chen, Tsai, Kun-Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper: Gerotarget (Focus on Aging)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905438/
https://www.ncbi.nlm.nih.gov/pubmed/26885898
http://dx.doi.org/10.18632/oncotarget.7387
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author Hung, Ching-Hsia
Chan, Shih-Hung
Chu, Pei-Ming
Lin, Huei-Chen
Tsai, Kun-Ling
author_facet Hung, Ching-Hsia
Chan, Shih-Hung
Chu, Pei-Ming
Lin, Huei-Chen
Tsai, Kun-Ling
author_sort Hung, Ching-Hsia
collection PubMed
description It is suggested that oxLDL is decisive in the initiation and development of atherosclerotic injuries. The up-regulation of oxidative stress and the generation of ROS act as key modulators in developing pro-atherosclerotic and anti-atherosclerotic processes in the human endothelial wall. In this present study, we confirmed that metformin enhanced SIRT1 and AMPK expression in human umbilical vein endothelial cells (HUVECs). Metformin also inhibited oxLDL-increased LOX-1 expression and oxLDL-collapsed AKT/eNOS levels. However, silencing SIRT1 and AMPK diminished the protective function of metformin against oxidative injuries. These results provide a new insight regarding the possible molecular mechanisms of metformin.
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spelling pubmed-49054382016-06-24 Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling Hung, Ching-Hsia Chan, Shih-Hung Chu, Pei-Ming Lin, Huei-Chen Tsai, Kun-Ling Oncotarget Research Paper: Gerotarget (Focus on Aging) It is suggested that oxLDL is decisive in the initiation and development of atherosclerotic injuries. The up-regulation of oxidative stress and the generation of ROS act as key modulators in developing pro-atherosclerotic and anti-atherosclerotic processes in the human endothelial wall. In this present study, we confirmed that metformin enhanced SIRT1 and AMPK expression in human umbilical vein endothelial cells (HUVECs). Metformin also inhibited oxLDL-increased LOX-1 expression and oxLDL-collapsed AKT/eNOS levels. However, silencing SIRT1 and AMPK diminished the protective function of metformin against oxidative injuries. These results provide a new insight regarding the possible molecular mechanisms of metformin. Impact Journals LLC 2016-02-14 /pmc/articles/PMC4905438/ /pubmed/26885898 http://dx.doi.org/10.18632/oncotarget.7387 Text en Copyright: © 2016 Hung et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Gerotarget (Focus on Aging)
Hung, Ching-Hsia
Chan, Shih-Hung
Chu, Pei-Ming
Lin, Huei-Chen
Tsai, Kun-Ling
Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling
title Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling
title_full Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling
title_fullStr Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling
title_full_unstemmed Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling
title_short Metformin regulates oxLDL-facilitated endothelial dysfunction by modulation of SIRT1 through repressing LOX-1-modulated oxidative signaling
title_sort metformin regulates oxldl-facilitated endothelial dysfunction by modulation of sirt1 through repressing lox-1-modulated oxidative signaling
topic Research Paper: Gerotarget (Focus on Aging)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905438/
https://www.ncbi.nlm.nih.gov/pubmed/26885898
http://dx.doi.org/10.18632/oncotarget.7387
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