PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches

The characterization of multigene families with high copy number variation is often approached through PCR amplification with highly degenerate primers to account for all expected variants flanking the region of interest. Such an approach often introduces PCR biases that result in an unbalanced repr...

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Detalles Bibliográficos
Autores principales: Marmesat, Elena, Soriano, Laura, Mazzoni, Camila J., Sommer, Simone, Godoy, José A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905633/
https://www.ncbi.nlm.nih.gov/pubmed/27294261
http://dx.doi.org/10.1371/journal.pone.0157402

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905633/
https://www.ncbi.nlm.nih.gov/pubmed/27294261
http://dx.doi.org/10.1371/journal.pone.0157402

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