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PCR Strategies for Complete Allele Calling in Multigene Families Using High-Throughput Sequencing Approaches
The characterization of multigene families with high copy number variation is often approached through PCR amplification with highly degenerate primers to account for all expected variants flanking the region of interest. Such an approach often introduces PCR biases that result in an unbalanced repr...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905633/ https://www.ncbi.nlm.nih.gov/pubmed/27294261 http://dx.doi.org/10.1371/journal.pone.0157402 |