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The Characterization of Escherichia coli CpdB as a Recombinant Protein Reveals that, besides Having the Expected 3´-Nucleotidase and 2´,3´-Cyclic Mononucleotide Phosphodiesterase Activities, It Is Also Active as Cyclic Dinucleotide Phosphodiesterase

Endogenous cyclic diadenylate phosphodiesterase activity was accidentally detected in lysates of Escherichia coli BL21. Since this kind of activity is uncommon in Gram-negative bacteria, its identification was undertaken. After partial purification and analysis by denaturing gel electrophoresis, ren...

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Detalles Bibliográficos
Autores principales: López-Villamizar, Iralis, Cabezas, Alicia, Pinto, Rosa María, Canales, José, Ribeiro, João Meireles, Cameselle, José Carlos, Costas, María Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4905662/
https://www.ncbi.nlm.nih.gov/pubmed/27294396
http://dx.doi.org/10.1371/journal.pone.0157308