Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens

The application of forward genetic screens to cultured human cells represents a powerful method to study gene function. The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disrupt gene function in mammalian cells, and has been applied to genome-wide screens. Here, we...

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Detalles Bibliográficos
Autores principales: Timms, Richard T., Menzies, Sam A., Tchasovnikarova, Iva A., Christensen, Lea C., Williamson, James C., Antrobus, Robin, Dougan, Gordon, Ellgaard, Lars, Lehner, Paul J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906395/
https://www.ncbi.nlm.nih.gov/pubmed/27283361
http://dx.doi.org/10.1038/ncomms11786

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906395/
https://www.ncbi.nlm.nih.gov/pubmed/27283361
http://dx.doi.org/10.1038/ncomms11786

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