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Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens
The application of forward genetic screens to cultured human cells represents a powerful method to study gene function. The repurposing of the bacterial CRISPR/Cas9 system provides an effective method to disrupt gene function in mammalian cells, and has been applied to genome-wide screens. Here, we...
Autores principales: | Timms, Richard T., Menzies, Sam A., Tchasovnikarova, Iva A., Christensen, Lea C., Williamson, James C., Antrobus, Robin, Dougan, Gordon, Ellgaard, Lars, Lehner, Paul J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906395/ https://www.ncbi.nlm.nih.gov/pubmed/27283361 http://dx.doi.org/10.1038/ncomms11786 |
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