Cargando…

Validation of an IFNγ/IL2 FluoroSpot assay for clinical trial monitoring

BACKGROUND: The FluoroSpot assay, an advancement of the ELISpot assay, enables simultaneous measurement of different analytes secreted at a single-cell level. This allows parallel detection of several cytokines secreted by immune cells upon antigen recognition. Easier standardization, higher sensiti...

Descripción completa

Detalles Bibliográficos
Autores principales:	Körber, Nina, Behrends, Uta, Hapfelmeier, Alexander, Protzer, Ulrike, Bauer, Tanja
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906590/ https://www.ncbi.nlm.nih.gov/pubmed/27297580 http://dx.doi.org/10.1186/s12967-016-0932-7

Ejemplares similares

Evaluation of T-activated proteins as recall antigens to monitor Epstein–Barr virus and human cytomegalovirus-specific T cells in a clinical trial setting
por: Körber, Nina, et al.
Publicado: (2020)

Triple Cytokine FluoroSpot Analysis of Human Antigen-Specific IFN-γ, IL-17A and IL-22 Responses
por: Dillenbeck, Tomas, et al.
Publicado: (2014)

Stepping up ELISpot: Multi-Level Analysis in FluoroSpot Assays
por: Janetzki, Sylvia, et al.
Publicado: (2014)

FluoroSpot Analysis of TLR-Activated Monocytes Reveals Several Distinct Cytokine-Secreting Subpopulations
por: Smedman, C, et al.
Publicado: (2012)

FluoroSpot assay to analyze SARS-CoV-2-specific T cell responses
por: Tiezzi, Camilla, et al.
Publicado: (2023)

Combined Antigen-Specific Interferon-γ and Interleukin-2 Release Assay (FluoroSpot) for the Diagnosis of Mycobacterium tuberculosis Infection
por: Chesov, Dumitru, et al.
Publicado: (2015)

Analysis of allelic cross-reactivity of monoclonal IgG antibodies by a multiplexed reverse FluoroSpot assay
por: Hoffmann-Veltung, Henriette, et al.
Publicado: (2022)

Four-Parameter FluoroSpot Assay Reveals That the Varicella Zoster Virus Elicits a Robust Memory T Cell IL-10 Response throughout Childhood
por: Nilsson, Anna, et al.
Publicado: (2022)

An evaluation of a FluoroSpot assay as a diagnostic tool to determine SARS-CoV-2 specific T cell responses
por: Mangsbo, Sara M., et al.
Publicado: (2021)

Evaluation of the dual IFNγ/IL-2 fluorospot-assay with flow cytometry for detection of HLA-restricted HIV-specific T-cell responses in HIV controllers
por: Keane, N, et al.
Publicado: (2012)

Utility of interferon gamma/tumor necrosis factor alpha FluoroSpot assay in differentiation between active tuberculosis and latent tuberculosis infection: a pilot study
por: Zhang, Lifan, et al.
Publicado: (2021)

Comparison of ELISpot and FluoroSpot in the Analysis of Swine Flu-Specific IgG and IgA Secretion by in Vivo Activated Human B Cells
por: Kesa, Gun, et al.
Publicado: (2012)

Immune monitoring technology primer: the enzyme-linked immunospot (Elispot) and Fluorospot assay
por: Janetzki, Sylvia
Publicado: (2015)

Memory B-Cell Responses Against Merozoite Antigens After Acute Plasmodium falciparum Malaria, Assessed Over One Year Using a Novel Multiplexed FluoroSpot Assay
por: Jahnmatz, Peter, et al.
Publicado: (2021)

A FluoroSpot B assay for the detection of IgA and IgG SARS-CoV-2 spike-specific memory B cells: Optimization and qualification for use in COVID-19 vaccine trials
por: Bisceglia, Hélène, et al.
Publicado: (2023)

An optimized IFN-γ ELISpot assay for the sensitive and standardized monitoring of CMV protein-reactive effector cells of cell-mediated immunity
por: Barabas, Sascha, et al.
Publicado: (2017)

Precision and linearity targets for validation of an IFNγ ELISPOT, cytokine flow cytometry, and tetramer assay using CMV peptides
por: Maecker, Holden T, et al.
Publicado: (2008)

Hepatitis B Vaccine Non-Responders Show Higher Frequencies of CD24(high)CD38(high) Regulatory B Cells and Lower Levels of IL-10 Expression Compared to Responders
por: Körber, Nina, et al.
Publicado: (2021)

Increased Level of IFN-γ and IL-4 Spot-Forming Cells on ELISPOT Assay as Biomarkers for Acute Graft-Versus-Host Disease and Concurrent Infections
por: Hirayama, Masahiro, et al.
Publicado: (2012)

IL-22 Is Mainly Produced by IFNγ-Secreting Cells but Is Dispensable for Host Protection against Mycobacterium tuberculosis Infection
por: Behrends, Jochen, et al.
Publicado: (2013)

Literature-Based Discovery of IFN-γ and Vaccine-Mediated Gene Interaction Networks
por: Özgür, Arzucan, et al.
Publicado: (2010)

Elevated serum IFN-γand IFN-γ/IL-6 ratio in Kikuchi-Fujimoto disease
por: He, Tingyan, et al.
Publicado: (2023)

IL-4, IL-13 and IFN-γ -induced genes in highly purified human neutrophils
por: Kummola, Laura, et al.
Publicado: (2023)

A modified method for rapid quantification of Chlamydia muridarum using Fluorospot
por: Keck, Jonathon, et al.
Publicado: (2019)

Simultaneous Detection of Antigen-Specific IgG- and IgM-Secreting Cells with a B Cell Fluorospot Assay
por: Bonsignori, Mattia, et al.
Publicado: (2012)

Generation of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems
por: Kugler, Franziska, et al.
Publicado: (2019)

A nested real-time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
por: Tran, Tuan M, et al.
Publicado: (2014)

The role of IL-12, IL-23 and IFN-γ in immunity to viruses
por: Novelli, Francesco, et al.
Publicado: (2004)

Development of a high-throughput γ-H2AX assay based on imaging flow cytometry
por: Lee, Younghyun, et al.
Publicado: (2019)

A simple cost-effective high performance liquid chromatographic assay of sulphadoxine in whole blood spotted on filter paper for field studies
por: Gbotosho, Grace O, et al.
Publicado: (2009)

Development and validation of a quantitative real-time polymerase chain assay for universal detection of the White Spot Syndrome Virus in marine crustaceans
por: Mendoza-Cano, Fernando, et al.
Publicado: (2013)

In Vivo Priming of Cd4 T Cells That Produce Interleukin (Il)-2 but Not IL-4 or Interferon (Ifn)-γ, and Can Subsequently Differentiate into IL-4–Or IFN-γ–Secreting Cells
por: Wang, Xiaowen, et al.
Publicado: (2001)

Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay - A comparative study
por: Buch, Karl, et al.
Publicado: (2012)

Dynamics of Intraocular IFN-γ, IL-17 and IL-10-Producing Cell Populations during Relapsing and Monophasic Rat Experimental Autoimmune Uveitis
por: Kaufmann, Ulrike, et al.
Publicado: (2012)

Publication Bias in Methodological Computational Research
por: Boulesteix, Anne-Laure, et al.
Publicado: (2015)

IFN-γ and CIITA modulate IL-6 expression in skeletal muscle
por: Adhikari, Abhinav, et al.
Publicado: (2020)

Human IL-23 is essential for IFN-γ-dependent immunity to mycobacteria
por: Philippot, Quentin, et al.
Publicado: (2023)

Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development
por: Mai, Elaine, et al.
Publicado: (2021)

Expression of genes related to inflammation – IL-6, IL-8, and IFN-γ in monitoring ustekinumab therapy: preliminary results
por: Kruszniewska-Rajs, Celina, et al.
Publicado: (2022)

Agarose spot migration assay to measure the chemoattractant potential of extracellular vesicles: applications in regenerative medicine and cancer metastasis
por: Clos-Sansalvador, Marta, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_c3thbhm7g9d2nbpru1gspud9rd