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Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing

BACKGROUND: The emergence of next-generation sequencing (NGS) technologies in the past decade has allowed the democratization of DNA sequencing both in terms of price per sequenced bases and ease to produce DNA libraries. When it comes to preparing DNA sequencing libraries for Illumina, the current...

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Detalles Bibliográficos
Autores principales: Aigrain, Louise, Gu, Yong, Quail, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4906846/
https://www.ncbi.nlm.nih.gov/pubmed/27297323
http://dx.doi.org/10.1186/s12864-016-2757-4