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Rapid Detection and Isolation of Escherichia coli O104:H4 from Milk Using Monoclonal Antibody-coated Magnetic Beads

Monoclonal antibodies (MAbs) specific for the lipopolysaccharide (LPS) of Escherichia coli O104:H4 were produced by fusion of Sp2/O-Ag-14 mouse myeloma cells with spleen cells of Balb/c mice, immunized with heat-inactivated and sonicated E. coli O104:H4 bacterial cells. Four MAbs specific for the E....

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Detalles Bibliográficos
Autores principales: Luciani, Mirella, Di Febo, Tiziana, Zilli, Katiuscia, Di Giannatale, Elisabetta, Armillotta, Gisella, Manna, Laura, Minelli, Fabio, Tittarelli, Manuela, Caprioli, Alfredo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4908104/
https://www.ncbi.nlm.nih.gov/pubmed/27379071
http://dx.doi.org/10.3389/fmicb.2016.00942