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A Modified Reverse One-Hybrid Screen Identifies Transcriptional Activation Domains in PHYTOCHROME-INTERACTING FACTOR 3

Transcriptional activation domains (TADs) are difficult to predict and identify, since they are not conserved and have little consensus. Here, we describe a yeast-based screening method that is able to identify individual amino acid residues involved in transcriptional activation in a high throughpu...

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Detalles Bibliográficos
Autores principales: Dalton, Jutta C., Bätz, Ulrike, Liu, Jason, Curie, Gemma L., Quail, Peter H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911399/
https://www.ncbi.nlm.nih.gov/pubmed/27379152
http://dx.doi.org/10.3389/fpls.2016.00881