Comparison of six commercial kits to extract bacterial chromosome and plasmid DNA for MiSeq sequencing

We compared commercial kits for extraction of genomic DNA from the Gram-negative bacterium Klebsiella pneumoniae for subsequent Miseq sequencing. Purification of DNA was based on matrix binding (silica or anion exchange resin) or differential precipitation (salting out), respectively. The choice of...

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Detalles Bibliográficos
Autores principales: Becker, Laura, Steglich, Matthias, Fuchs, Stephan, Werner, Guido, Nübel, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911584/
https://www.ncbi.nlm.nih.gov/pubmed/27312200
http://dx.doi.org/10.1038/srep28063

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4911584/
https://www.ncbi.nlm.nih.gov/pubmed/27312200
http://dx.doi.org/10.1038/srep28063

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