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Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage
The GTPase RhoA promotes contractile ring assembly and furrow ingression during cytokinesis. Although many factors that regulate RhoA during cytokinesis have been characterized, the spatiotemporal regulatory logic remains undefined. We have developed an optogenetic probe to gain tight spatial and te...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915195/ https://www.ncbi.nlm.nih.gov/pubmed/27298323 http://dx.doi.org/10.1083/jcb.201603025 |
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author | Wagner, Elizabeth Glotzer, Michael |
author_facet | Wagner, Elizabeth Glotzer, Michael |
author_sort | Wagner, Elizabeth |
collection | PubMed |
description | The GTPase RhoA promotes contractile ring assembly and furrow ingression during cytokinesis. Although many factors that regulate RhoA during cytokinesis have been characterized, the spatiotemporal regulatory logic remains undefined. We have developed an optogenetic probe to gain tight spatial and temporal control of RhoA activity in mammalian cells and demonstrate that cytokinetic furrowing is primarily regulated at the level of RhoA activation. Light-mediated recruitment of a RhoGEF domain to the plasma membrane leads to rapid induction of RhoA activity, leading to assembly of cytokinetic furrows that partially ingress. Furthermore, furrow formation in response to RhoA activation is not temporally or spatially restricted. RhoA activation is sufficient to generate furrows at both the cell equator and cell poles, in both metaphase and anaphase. Remarkably, furrow formation can be initiated in rounded interphase cells, but not adherent cells. These results indicate that RhoA activation is sufficient to induce assembly of functional contractile rings and that cell rounding facilitates furrow formation. |
format | Online Article Text |
id | pubmed-4915195 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-49151952016-12-20 Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage Wagner, Elizabeth Glotzer, Michael J Cell Biol Research Articles The GTPase RhoA promotes contractile ring assembly and furrow ingression during cytokinesis. Although many factors that regulate RhoA during cytokinesis have been characterized, the spatiotemporal regulatory logic remains undefined. We have developed an optogenetic probe to gain tight spatial and temporal control of RhoA activity in mammalian cells and demonstrate that cytokinetic furrowing is primarily regulated at the level of RhoA activation. Light-mediated recruitment of a RhoGEF domain to the plasma membrane leads to rapid induction of RhoA activity, leading to assembly of cytokinetic furrows that partially ingress. Furthermore, furrow formation in response to RhoA activation is not temporally or spatially restricted. RhoA activation is sufficient to generate furrows at both the cell equator and cell poles, in both metaphase and anaphase. Remarkably, furrow formation can be initiated in rounded interphase cells, but not adherent cells. These results indicate that RhoA activation is sufficient to induce assembly of functional contractile rings and that cell rounding facilitates furrow formation. The Rockefeller University Press 2016-06-20 /pmc/articles/PMC4915195/ /pubmed/27298323 http://dx.doi.org/10.1083/jcb.201603025 Text en © 2016 Wagner and Glotzer This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Wagner, Elizabeth Glotzer, Michael Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage |
title | Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage |
title_full | Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage |
title_fullStr | Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage |
title_full_unstemmed | Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage |
title_short | Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage |
title_sort | local rhoa activation induces cytokinetic furrows independent of spindle position and cell cycle stage |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4915195/ https://www.ncbi.nlm.nih.gov/pubmed/27298323 http://dx.doi.org/10.1083/jcb.201603025 |
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