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The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP
Bacteriophytochromes are promising tools for tissue microscopy and imaging due to their fluorescence in the near-infrared region. These applications require optimization of the originally low fluorescence quantum yields via genetic engineering. Factors that favour fluorescence over other non-radiati...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4916461/ https://www.ncbi.nlm.nih.gov/pubmed/27329837 http://dx.doi.org/10.1038/srep28444 |