Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5

A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results re...

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Autores principales: Mukram, Ismailsab, Ramesh, Masarbo, Monisha, T. R., Nayak, Anand S., Karegoudar, T. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917499/
https://www.ncbi.nlm.nih.gov/pubmed/28330213
http://dx.doi.org/10.1007/s13205-016-0456-0
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author Mukram, Ismailsab
Ramesh, Masarbo
Monisha, T. R.
Nayak, Anand S.
Karegoudar, T. B.
author_facet Mukram, Ismailsab
Ramesh, Masarbo
Monisha, T. R.
Nayak, Anand S.
Karegoudar, T. B.
author_sort Mukram, Ismailsab
collection PubMed
description A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results reveal that 100, 98, and 88 % degradation was achieved for 2, 2.5, and 3 % butyronitrile, respectively. The strain is capable of growing in as high as 5 % butyronitrile concentration. A two-step pathway involving nitrile hydratase (NHase) and amidase was observed for the biodegradation of butyronitrile. Complete degradation (mineralization) of butyronitrile with the help of metabolite feeding experiment was reported. The significance of this investigation was the capability of the strain to completely degrade and its ability to grow on higher concentrations of butyronitrile. These potential features make it a suitable candidate for practical field application for effective in situ bioremediation of butyronitrile contaminated sites.
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spelling pubmed-49174992016-06-23 Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5 Mukram, Ismailsab Ramesh, Masarbo Monisha, T. R. Nayak, Anand S. Karegoudar, T. B. 3 Biotech Original Article A nitrile utilizing bacterium Rhodococcus sp. MTB5 was previously isolated in our laboratory by the enrichment culture technique. It is able to utilize butyronitrile as sole carbon, nitrogen, and energy source. Maximum butyronitrile degrading property of this strain has been investigated. Results reveal that 100, 98, and 88 % degradation was achieved for 2, 2.5, and 3 % butyronitrile, respectively. The strain is capable of growing in as high as 5 % butyronitrile concentration. A two-step pathway involving nitrile hydratase (NHase) and amidase was observed for the biodegradation of butyronitrile. Complete degradation (mineralization) of butyronitrile with the help of metabolite feeding experiment was reported. The significance of this investigation was the capability of the strain to completely degrade and its ability to grow on higher concentrations of butyronitrile. These potential features make it a suitable candidate for practical field application for effective in situ bioremediation of butyronitrile contaminated sites. Springer Berlin Heidelberg 2016-06-22 2016-12 /pmc/articles/PMC4917499/ /pubmed/28330213 http://dx.doi.org/10.1007/s13205-016-0456-0 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Mukram, Ismailsab
Ramesh, Masarbo
Monisha, T. R.
Nayak, Anand S.
Karegoudar, T. B.
Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_full Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_fullStr Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_full_unstemmed Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_short Biodegradation of butyronitrile and demonstration of its mineralization by Rhodococcus sp. MTB5
title_sort biodegradation of butyronitrile and demonstration of its mineralization by rhodococcus sp. mtb5
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917499/
https://www.ncbi.nlm.nih.gov/pubmed/28330213
http://dx.doi.org/10.1007/s13205-016-0456-0
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