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A novel mechanism for direct real-time polymerase chain reaction that does not require DNA isolation from prokaryotic cells
Typically, polymerase chain reaction (PCR) is performed after DNA isolation. Real-time PCR (qPCR), also known as direct qPCR in mammalian cells with weak membranes, is a common technique using crude samples subjected to preliminary boiling to elute DNA. However, applying this methodology to prokaryo...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4917819/ https://www.ncbi.nlm.nih.gov/pubmed/27334801 http://dx.doi.org/10.1038/srep28000 |