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Mutation assay using single-molecule real-time (SMRT(TM)) sequencing technology
INTRODUCTION: We present here a simple, phenotype-independent mutation assay using a PacBio RSII DNA sequencer employing single-molecule real-time (SMRT) sequencing technology. Salmonella typhimurium YG7108 was treated with the alkylating agent N-ethyl-N-nitrosourea (ENU) and grown though several ge...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918037/ https://www.ncbi.nlm.nih.gov/pubmed/27350811 http://dx.doi.org/10.1186/s41021-015-0017-5 |