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Mutation assay using single-molecule real-time (SMRT(TM)) sequencing technology

INTRODUCTION: We present here a simple, phenotype-independent mutation assay using a PacBio RSII DNA sequencer employing single-molecule real-time (SMRT) sequencing technology. Salmonella typhimurium YG7108 was treated with the alkylating agent N-ethyl-N-nitrosourea (ENU) and grown though several ge...

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Detalles Bibliográficos
Autores principales:	Matsuda, Tomonari, Matsuda, Shun, Yamada, Masami
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918037/ https://www.ncbi.nlm.nih.gov/pubmed/27350811 http://dx.doi.org/10.1186/s41021-015-0017-5

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