Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)

We present a high content multiwell plate cell-based assay approach to quantify protein interactions directly in cells using Förster resonance energy transfer (FRET) read out by automated fluorescence lifetime imaging (FLIM). Automated FLIM is implemented using wide-field time-gated detection, typic...

Descripción completa

Detalles Bibliográficos
Autores principales: Margineanu, Anca, Chan, Jia Jia, Kelly, Douglas J., Warren, Sean C., Flatters, Delphine, Kumar, Sunil, Katan, Matilda, Dunsby, Christopher W., French, Paul M. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919659/
https://www.ncbi.nlm.nih.gov/pubmed/27339025
http://dx.doi.org/10.1038/srep28186

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919659/
https://www.ncbi.nlm.nih.gov/pubmed/27339025
http://dx.doi.org/10.1038/srep28186

Ejemplares similares