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Screening for protein-protein interactions using Förster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM)

We present a high content multiwell plate cell-based assay approach to quantify protein interactions directly in cells using Förster resonance energy transfer (FRET) read out by automated fluorescence lifetime imaging (FLIM). Automated FLIM is implemented using wide-field time-gated detection, typic...

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Detalles Bibliográficos
Autores principales:	Margineanu, Anca, Chan, Jia Jia, Kelly, Douglas J., Warren, Sean C., Flatters, Delphine, Kumar, Sunil, Katan, Matilda, Dunsby, Christopher W., French, Paul M. W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4919659/ https://www.ncbi.nlm.nih.gov/pubmed/27339025 http://dx.doi.org/10.1038/srep28186

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