Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo
Gallbladder carcinoma (GBC) is a highly lethal malignancy of the gastrointestinal tract. Despite extensive research, the underlying molecular mechanism of GBC remains largely unclear. Stathmin 1 (STMN1) is an important cytosolic protein associated with microtubule stability that was reported to be i...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923895/ https://www.ncbi.nlm.nih.gov/pubmed/27349455 http://dx.doi.org/10.1038/srep28833 |
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author | Wang, Jiwen Yao, Yanli Ming, Yue Shen, Sheng Wu, Nan Liu, Jiaqi Liu, Han Suo, Tao Pan, Hongtao Zhang, Dexiang Ding, Kan Liu, Houbao |
author_facet | Wang, Jiwen Yao, Yanli Ming, Yue Shen, Sheng Wu, Nan Liu, Jiaqi Liu, Han Suo, Tao Pan, Hongtao Zhang, Dexiang Ding, Kan Liu, Houbao |
author_sort | Wang, Jiwen |
collection | PubMed |
description | Gallbladder carcinoma (GBC) is a highly lethal malignancy of the gastrointestinal tract. Despite extensive research, the underlying molecular mechanism of GBC remains largely unclear. Stathmin 1 (STMN1) is an important cytosolic protein associated with microtubule stability that was reported to be involved in tumorigenesis. Up to our knowledge, its role in gallbladder carcinoma has not been analyzed. In this study, we found that STMN1 was significantly highly expressed in GBC by immunohistochemistry (IHC). Further research demonstrated that silencing of STMN1 inhibited cell growth in vitro. Moreover, knockdown of STMN1 induced apoptosis and delayed G2/M phase transformation in GBC cells. Our data support a rationale for further studies that the silencing of STMN1 may regulate the activity of p38 MAPK kinase and p53/p21 signal pathway. Besides, xenografted gallbladder carcinoma cells growth were significantly impaired after STMN1 was silenced in vivo. These results suggested that STMN1 played an important role in cell proliferation and migration. This provided a potential clue for investigating the therapeutic target in GBC. |
format | Online Article Text |
id | pubmed-4923895 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49238952016-06-28 Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo Wang, Jiwen Yao, Yanli Ming, Yue Shen, Sheng Wu, Nan Liu, Jiaqi Liu, Han Suo, Tao Pan, Hongtao Zhang, Dexiang Ding, Kan Liu, Houbao Sci Rep Article Gallbladder carcinoma (GBC) is a highly lethal malignancy of the gastrointestinal tract. Despite extensive research, the underlying molecular mechanism of GBC remains largely unclear. Stathmin 1 (STMN1) is an important cytosolic protein associated with microtubule stability that was reported to be involved in tumorigenesis. Up to our knowledge, its role in gallbladder carcinoma has not been analyzed. In this study, we found that STMN1 was significantly highly expressed in GBC by immunohistochemistry (IHC). Further research demonstrated that silencing of STMN1 inhibited cell growth in vitro. Moreover, knockdown of STMN1 induced apoptosis and delayed G2/M phase transformation in GBC cells. Our data support a rationale for further studies that the silencing of STMN1 may regulate the activity of p38 MAPK kinase and p53/p21 signal pathway. Besides, xenografted gallbladder carcinoma cells growth were significantly impaired after STMN1 was silenced in vivo. These results suggested that STMN1 played an important role in cell proliferation and migration. This provided a potential clue for investigating the therapeutic target in GBC. Nature Publishing Group 2016-06-28 /pmc/articles/PMC4923895/ /pubmed/27349455 http://dx.doi.org/10.1038/srep28833 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, Jiwen Yao, Yanli Ming, Yue Shen, Sheng Wu, Nan Liu, Jiaqi Liu, Han Suo, Tao Pan, Hongtao Zhang, Dexiang Ding, Kan Liu, Houbao Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo |
title | Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo |
title_full | Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo |
title_fullStr | Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo |
title_full_unstemmed | Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo |
title_short | Downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo |
title_sort | downregulation of stathmin 1 in human gallbladder carcinoma inhibits tumor growth in vitro and in vivo |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4923895/ https://www.ncbi.nlm.nih.gov/pubmed/27349455 http://dx.doi.org/10.1038/srep28833 |
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