Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation

B-cell specific moloney leukemia virus insertion site 1 (Bmi-1) gene plays important roles in gastric cancer, but the epigenetic regulatory mechanism by microRNA (miRNA) and the functional significance of Bmi-1 inhibition in gastric cancer remains elusive. In this study, we systematically investigat...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Changping, Zheng, Xiao, Li, Xiaodong, Fesler, Andrew, Hu, Wenwei, Chen, Lujun, Xu, Bin, Wang, Qi, Tong, Anthony, Burke, Stephanie, Ju, Jingfang, Jiang, Jingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4924733/
https://www.ncbi.nlm.nih.gov/pubmed/26894855
http://dx.doi.org/10.18632/oncotarget.7392
_version_ 1782439912863170560
author Wu, Changping
Zheng, Xiao
Li, Xiaodong
Fesler, Andrew
Hu, Wenwei
Chen, Lujun
Xu, Bin
Wang, Qi
Tong, Anthony
Burke, Stephanie
Ju, Jingfang
Jiang, Jingting
author_facet Wu, Changping
Zheng, Xiao
Li, Xiaodong
Fesler, Andrew
Hu, Wenwei
Chen, Lujun
Xu, Bin
Wang, Qi
Tong, Anthony
Burke, Stephanie
Ju, Jingfang
Jiang, Jingting
author_sort Wu, Changping
collection PubMed
description B-cell specific moloney leukemia virus insertion site 1 (Bmi-1) gene plays important roles in gastric cancer, but the epigenetic regulatory mechanism by microRNA (miRNA) and the functional significance of Bmi-1 inhibition in gastric cancer remains elusive. In this study, we systematically investigated the functional roles of miRNA mediated Bmi-1 suppression in gastric cancer. Our results show that the expression of miR-15a is significantly reduced in gastric cancer and the protein expression levels of Bmi-1 are inversely correlated with miR-15a (P = 0.034) in gastric cancer patient samples. Functional studies revealed that ectopic expression of miR-15a decreased Bmi-1 in gastric cancer cell lines with reduced proliferation and tumor invasion. High levels of Bmi-1 in gastric cancer patients are significantly associated with better overall survival (P = 0.024) based on the Kaplan-Meier survival analysis.
format Online
Article
Text
id pubmed-4924733
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Impact Journals LLC
record_format MEDLINE/PubMed
spelling pubmed-49247332016-07-13 Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation Wu, Changping Zheng, Xiao Li, Xiaodong Fesler, Andrew Hu, Wenwei Chen, Lujun Xu, Bin Wang, Qi Tong, Anthony Burke, Stephanie Ju, Jingfang Jiang, Jingting Oncotarget Research Paper B-cell specific moloney leukemia virus insertion site 1 (Bmi-1) gene plays important roles in gastric cancer, but the epigenetic regulatory mechanism by microRNA (miRNA) and the functional significance of Bmi-1 inhibition in gastric cancer remains elusive. In this study, we systematically investigated the functional roles of miRNA mediated Bmi-1 suppression in gastric cancer. Our results show that the expression of miR-15a is significantly reduced in gastric cancer and the protein expression levels of Bmi-1 are inversely correlated with miR-15a (P = 0.034) in gastric cancer patient samples. Functional studies revealed that ectopic expression of miR-15a decreased Bmi-1 in gastric cancer cell lines with reduced proliferation and tumor invasion. High levels of Bmi-1 in gastric cancer patients are significantly associated with better overall survival (P = 0.024) based on the Kaplan-Meier survival analysis. Impact Journals LLC 2016-02-15 /pmc/articles/PMC4924733/ /pubmed/26894855 http://dx.doi.org/10.18632/oncotarget.7392 Text en Copyright: © 2016 Wu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wu, Changping
Zheng, Xiao
Li, Xiaodong
Fesler, Andrew
Hu, Wenwei
Chen, Lujun
Xu, Bin
Wang, Qi
Tong, Anthony
Burke, Stephanie
Ju, Jingfang
Jiang, Jingting
Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation
title Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation
title_full Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation
title_fullStr Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation
title_full_unstemmed Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation
title_short Reduction of gastric cancer proliferation and invasion by miR-15a mediated suppression of Bmi-1 translation
title_sort reduction of gastric cancer proliferation and invasion by mir-15a mediated suppression of bmi-1 translation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4924733/
https://www.ncbi.nlm.nih.gov/pubmed/26894855
http://dx.doi.org/10.18632/oncotarget.7392
work_keys_str_mv AT wuchangping reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT zhengxiao reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT lixiaodong reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT feslerandrew reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT huwenwei reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT chenlujun reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT xubin reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT wangqi reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT tonganthony reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT burkestephanie reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT jujingfang reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation
AT jiangjingting reductionofgastriccancerproliferationandinvasionbymir15amediatedsuppressionofbmi1translation

Ejemplares similares