Shotgun Proteomics of Tomato Fruits: Evaluation, Optimization and Validation of Sample Preparation Methods and Mass Spectrometric Parameters

An optimized protocol was developed for shotgun proteomics of tomato fruit, which is a recalcitrant tissue due to a high percentage of sugars and secondary metabolites. A number of protein extraction and fractionation techniques were examined for optimal protein extraction from tomato fruits followe...

Descripción completa

Detalles Bibliográficos
Autores principales: Kilambi, Himabindu V., Manda, Kalyani, Sanivarapu, Hemalatha, Maurya, Vineet K., Sharma, Rameshwar, Sreelakshmi, Yellamaraju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925719/
https://www.ncbi.nlm.nih.gov/pubmed/27446192
http://dx.doi.org/10.3389/fpls.2016.00969

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925719/
https://www.ncbi.nlm.nih.gov/pubmed/27446192
http://dx.doi.org/10.3389/fpls.2016.00969

Ejemplares similares