Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites

Mammalian ageing features biological attrition evident at cellular, genetic and epigenetic levels. Mutation of mitochondrial DNA, and nuclear DNA methylation changes are well established correlates of ageing. The methylation of mitochondrial DNA (mtDNA) is a new and incompletely described phenomenon...

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Autores principales: Mawlood, Shakhawan K., Dennany, Lynn, Watson, Nigel, Dempster, John, Pickard, Benjamin S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925819/
https://www.ncbi.nlm.nih.gov/pubmed/26887692
http://dx.doi.org/10.18632/aging.100892
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author Mawlood, Shakhawan K.
Dennany, Lynn
Watson, Nigel
Dempster, John
Pickard, Benjamin S.
author_facet Mawlood, Shakhawan K.
Dennany, Lynn
Watson, Nigel
Dempster, John
Pickard, Benjamin S.
author_sort Mawlood, Shakhawan K.
collection PubMed
description Mammalian ageing features biological attrition evident at cellular, genetic and epigenetic levels. Mutation of mitochondrial DNA, and nuclear DNA methylation changes are well established correlates of ageing. The methylation of mitochondrial DNA (mtDNA) is a new and incompletely described phenomenon with unknown biological control and significance. Here we describe the bisulphite sequencing of mtDNA from 82 individuals aged 18‐91 years. We detected low and variable levels of mtDNA methylation at 54 of 133 CpG sites interrogated. Regression analysis of methylation levels at two CpG sites (M1215 and M1313) located within the 12S ribosomal RNA gene showed an inverse correlation with subject age suggesting their utility as epigenetic markers of ageing.
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spelling pubmed-49258192016-07-01 Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites Mawlood, Shakhawan K. Dennany, Lynn Watson, Nigel Dempster, John Pickard, Benjamin S. Aging (Albany NY) Research Paper Mammalian ageing features biological attrition evident at cellular, genetic and epigenetic levels. Mutation of mitochondrial DNA, and nuclear DNA methylation changes are well established correlates of ageing. The methylation of mitochondrial DNA (mtDNA) is a new and incompletely described phenomenon with unknown biological control and significance. Here we describe the bisulphite sequencing of mtDNA from 82 individuals aged 18‐91 years. We detected low and variable levels of mtDNA methylation at 54 of 133 CpG sites interrogated. Regression analysis of methylation levels at two CpG sites (M1215 and M1313) located within the 12S ribosomal RNA gene showed an inverse correlation with subject age suggesting their utility as epigenetic markers of ageing. Impact Journals LLC 2016-02-17 /pmc/articles/PMC4925819/ /pubmed/26887692 http://dx.doi.org/10.18632/aging.100892 Text en Copyright: © 2016 Mawlood et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Mawlood, Shakhawan K.
Dennany, Lynn
Watson, Nigel
Dempster, John
Pickard, Benjamin S.
Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites
title Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites
title_full Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites
title_fullStr Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites
title_full_unstemmed Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites
title_short Quantification of global mitochondrial DNA methylation levels and inverse correlation with age at two CpG sites
title_sort quantification of global mitochondrial dna methylation levels and inverse correlation with age at two cpg sites
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4925819/
https://www.ncbi.nlm.nih.gov/pubmed/26887692
http://dx.doi.org/10.18632/aging.100892
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