Identification of Vimentin as a Potential Therapeutic Target against HIV Infection
A combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART has markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvant...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926169/ https://www.ncbi.nlm.nih.gov/pubmed/27314381 http://dx.doi.org/10.3390/v8060098 |
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author | Fernández-Ortega, Celia Ramírez, Anna Casillas, Dionne Paneque, Taimi Ubieta, Raimundo Dubed, Marta Navea, Leonor Castellanos-Serra, Lila Duarte, Carlos Falcon, Viviana Reyes, Osvaldo Garay, Hilda Silva, Eladio Noa, Enrique Ramos, Yassel Besada, Vladimir Betancourt, Lázaro |
author_facet | Fernández-Ortega, Celia Ramírez, Anna Casillas, Dionne Paneque, Taimi Ubieta, Raimundo Dubed, Marta Navea, Leonor Castellanos-Serra, Lila Duarte, Carlos Falcon, Viviana Reyes, Osvaldo Garay, Hilda Silva, Eladio Noa, Enrique Ramos, Yassel Besada, Vladimir Betancourt, Lázaro |
author_sort | Fernández-Ortega, Celia |
collection | PubMed |
description | A combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART has markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvantage, resistance development, remains to be solved. Hope is focused on developing drugs against cellular targets. This strategy is expected to prevent the emergence of viral resistance. In this study, using a comparative proteomic approach in MT4 cells treated with an anti-HIV leukocyte extract, we identified vimentin, a molecule forming intermediate filaments in the cell, as a possible target against HIV infection. We demonstrated a strong reduction of an HIV-1 based lentivirus expressing the enhanced green fluorescent protein (eGFP) in vimentin knockdown cells, and a noteworthy decrease of HIV-1 capsid protein antigen (CAp24) in those cells using a multiround infectivity assay. Electron micrographs showed changes in the structure of intermediate filaments when MT4 cells were treated with an anti-HIV leukocyte extract. Changes in the structure of intermediate filaments were also observed in vimentin knockdown MT4 cells. A synthetic peptide derived from a cytoskeleton protein showed potent inhibitory activity on HIV-1 infection, and low cytotoxicity. Our data suggest that vimentin can be a suitable target to inhibit HIV-1. |
format | Online Article Text |
id | pubmed-4926169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-49261692016-07-06 Identification of Vimentin as a Potential Therapeutic Target against HIV Infection Fernández-Ortega, Celia Ramírez, Anna Casillas, Dionne Paneque, Taimi Ubieta, Raimundo Dubed, Marta Navea, Leonor Castellanos-Serra, Lila Duarte, Carlos Falcon, Viviana Reyes, Osvaldo Garay, Hilda Silva, Eladio Noa, Enrique Ramos, Yassel Besada, Vladimir Betancourt, Lázaro Viruses Article A combination of antiviral drugs known as antiretroviral therapy (ART) has shown effectiveness against the human immunodeficiency virus (HIV). ART has markedly decreased mortality and morbidity among HIV-infected patients, having even reduced HIV transmission. However, an important current disadvantage, resistance development, remains to be solved. Hope is focused on developing drugs against cellular targets. This strategy is expected to prevent the emergence of viral resistance. In this study, using a comparative proteomic approach in MT4 cells treated with an anti-HIV leukocyte extract, we identified vimentin, a molecule forming intermediate filaments in the cell, as a possible target against HIV infection. We demonstrated a strong reduction of an HIV-1 based lentivirus expressing the enhanced green fluorescent protein (eGFP) in vimentin knockdown cells, and a noteworthy decrease of HIV-1 capsid protein antigen (CAp24) in those cells using a multiround infectivity assay. Electron micrographs showed changes in the structure of intermediate filaments when MT4 cells were treated with an anti-HIV leukocyte extract. Changes in the structure of intermediate filaments were also observed in vimentin knockdown MT4 cells. A synthetic peptide derived from a cytoskeleton protein showed potent inhibitory activity on HIV-1 infection, and low cytotoxicity. Our data suggest that vimentin can be a suitable target to inhibit HIV-1. MDPI 2016-06-15 /pmc/articles/PMC4926169/ /pubmed/27314381 http://dx.doi.org/10.3390/v8060098 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Fernández-Ortega, Celia Ramírez, Anna Casillas, Dionne Paneque, Taimi Ubieta, Raimundo Dubed, Marta Navea, Leonor Castellanos-Serra, Lila Duarte, Carlos Falcon, Viviana Reyes, Osvaldo Garay, Hilda Silva, Eladio Noa, Enrique Ramos, Yassel Besada, Vladimir Betancourt, Lázaro Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
title | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
title_full | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
title_fullStr | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
title_full_unstemmed | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
title_short | Identification of Vimentin as a Potential Therapeutic Target against HIV Infection |
title_sort | identification of vimentin as a potential therapeutic target against hiv infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926169/ https://www.ncbi.nlm.nih.gov/pubmed/27314381 http://dx.doi.org/10.3390/v8060098 |
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