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In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts

Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cu...

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Detalles Bibliográficos
Autores principales: Owen, Carolyn A., Moukarzel, Romy, Huang, Xiao, Kassem, Mona A., Eliasco, Eleonora, Aranda, Miguel A., Coutts, Robert H. A., Livieratos, Ioannis C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926190/
https://www.ncbi.nlm.nih.gov/pubmed/27314380
http://dx.doi.org/10.3390/v8060170