Studies to Prevent Degradation of Recombinant Fc-Fusion Protein Expressed in Mammalian Cell Line and Protein Characterization

Clipping of recombinant proteins is a major issue in animal cell cultures. A recombinant Fc-fusion protein, VEGFR1(D1–D3)-Fc expressed in CHOK1SV GS-KO cells was observed to be undergoing clippings in lab scale cultures. Partial cleaving of expressed protein initiated early on in cell culture and wa...

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Detalles Bibliográficos
Autores principales: Chakrabarti, Sanjukta, Barrow, Colin J., Kanwar, Rupinder K., Ramana, Venkata, Kanwar, Jagat R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926446/
https://www.ncbi.nlm.nih.gov/pubmed/27294920
http://dx.doi.org/10.3390/ijms17060913

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926446/
https://www.ncbi.nlm.nih.gov/pubmed/27294920
http://dx.doi.org/10.3390/ijms17060913

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