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Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels
Hepatitis C virus (HCV) substantially affects lipid metabolism, and remodeling of sphingolipids appears to be essential for HCV persistence in vitro. The aim of the current study is the evaluation of serum sphingolipid variations during acute HCV infection. We enrolled prospectively 60 consecutive p...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926455/ https://www.ncbi.nlm.nih.gov/pubmed/27304952 http://dx.doi.org/10.3390/ijms17060922 |
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author | Grammatikos, Georgios Dietz, Julia Ferreiros, Nerea Koch, Alexander Dultz, Georg Bon, Dimitra Karakasiliotis, Ioannis Lutz, Thomas Knecht, Gaby Gute, Peter Herrmann, Eva Zeuzem, Stefan Mavromara, Penelope Sarrazin, Christoph Pfeilschifter, Josef |
author_facet | Grammatikos, Georgios Dietz, Julia Ferreiros, Nerea Koch, Alexander Dultz, Georg Bon, Dimitra Karakasiliotis, Ioannis Lutz, Thomas Knecht, Gaby Gute, Peter Herrmann, Eva Zeuzem, Stefan Mavromara, Penelope Sarrazin, Christoph Pfeilschifter, Josef |
author_sort | Grammatikos, Georgios |
collection | PubMed |
description | Hepatitis C virus (HCV) substantially affects lipid metabolism, and remodeling of sphingolipids appears to be essential for HCV persistence in vitro. The aim of the current study is the evaluation of serum sphingolipid variations during acute HCV infection. We enrolled prospectively 60 consecutive patients with acute HCV infection, most of them already infected with human immunodeficiency virus (HIV), and serum was collected at the time of diagnosis and longitudinally over a six-month period until initiation of antiviral therapy or confirmed spontaneous clearance. Quantification of serum sphingolipids was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Spontaneous clearance was observed in 11 out of 60 patients (18.3%), a sustained viral response (SVR) in 43 out of 45 patients (95.5%) receiving an antiviral treatment after follow-up, whereas persistence of HCV occurred in six out of 60 patients (10%). C24-ceramide (C24-Cer)-levels increased at follow-up in patients with spontaneous HCV eradication (p < 0.01), as compared to baseline. Sphingosine and sphinganine values were significantly upregulated in patients unable to clear HCV over time compared to patients with spontaneous clearance of HCV infection on follow-up (p = 0.013 and 0.006, respectively). In summary, the persistence of HCV after acute infection induces a downregulation of C24Cer and a simultaneous elevation of serum sphingosine and sphinganine concentrations. |
format | Online Article Text |
id | pubmed-4926455 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-49264552016-07-06 Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels Grammatikos, Georgios Dietz, Julia Ferreiros, Nerea Koch, Alexander Dultz, Georg Bon, Dimitra Karakasiliotis, Ioannis Lutz, Thomas Knecht, Gaby Gute, Peter Herrmann, Eva Zeuzem, Stefan Mavromara, Penelope Sarrazin, Christoph Pfeilschifter, Josef Int J Mol Sci Article Hepatitis C virus (HCV) substantially affects lipid metabolism, and remodeling of sphingolipids appears to be essential for HCV persistence in vitro. The aim of the current study is the evaluation of serum sphingolipid variations during acute HCV infection. We enrolled prospectively 60 consecutive patients with acute HCV infection, most of them already infected with human immunodeficiency virus (HIV), and serum was collected at the time of diagnosis and longitudinally over a six-month period until initiation of antiviral therapy or confirmed spontaneous clearance. Quantification of serum sphingolipids was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Spontaneous clearance was observed in 11 out of 60 patients (18.3%), a sustained viral response (SVR) in 43 out of 45 patients (95.5%) receiving an antiviral treatment after follow-up, whereas persistence of HCV occurred in six out of 60 patients (10%). C24-ceramide (C24-Cer)-levels increased at follow-up in patients with spontaneous HCV eradication (p < 0.01), as compared to baseline. Sphingosine and sphinganine values were significantly upregulated in patients unable to clear HCV over time compared to patients with spontaneous clearance of HCV infection on follow-up (p = 0.013 and 0.006, respectively). In summary, the persistence of HCV after acute infection induces a downregulation of C24Cer and a simultaneous elevation of serum sphingosine and sphinganine concentrations. MDPI 2016-06-13 /pmc/articles/PMC4926455/ /pubmed/27304952 http://dx.doi.org/10.3390/ijms17060922 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Grammatikos, Georgios Dietz, Julia Ferreiros, Nerea Koch, Alexander Dultz, Georg Bon, Dimitra Karakasiliotis, Ioannis Lutz, Thomas Knecht, Gaby Gute, Peter Herrmann, Eva Zeuzem, Stefan Mavromara, Penelope Sarrazin, Christoph Pfeilschifter, Josef Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels |
title | Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels |
title_full | Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels |
title_fullStr | Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels |
title_full_unstemmed | Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels |
title_short | Persistence of HCV in Acutely-Infected Patients Depletes C24-Ceramide and Upregulates Sphingosine and Sphinganine Serum Levels |
title_sort | persistence of hcv in acutely-infected patients depletes c24-ceramide and upregulates sphingosine and sphinganine serum levels |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926455/ https://www.ncbi.nlm.nih.gov/pubmed/27304952 http://dx.doi.org/10.3390/ijms17060922 |
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