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Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow
Endomitosis is a special type of mitosis in which only cytokinesis—the final step of the cell division cycle—is defective, resulting in polyploid cells. Although endomitosis is biologically important, its regulatory aspects remain elusive. Psychosine, a lysogalactosylceramide, prevents proper cytoki...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927278/ https://www.ncbi.nlm.nih.gov/pubmed/27170180 http://dx.doi.org/10.1091/mbc.E15-08-0555 |
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author | Watanabe, Hiroshi Okahara, Kyohei Naito-Matsui, Yuko Abe, Mitsuhiro Go, Shinji Inokuchi, Jinichi Okazaki, Toshiro Kobayashi, Toshihide Kozutsumi, Yasunori Oka, Shogo Takematsu, Hiromu |
author_facet | Watanabe, Hiroshi Okahara, Kyohei Naito-Matsui, Yuko Abe, Mitsuhiro Go, Shinji Inokuchi, Jinichi Okazaki, Toshiro Kobayashi, Toshihide Kozutsumi, Yasunori Oka, Shogo Takematsu, Hiromu |
author_sort | Watanabe, Hiroshi |
collection | PubMed |
description | Endomitosis is a special type of mitosis in which only cytokinesis—the final step of the cell division cycle—is defective, resulting in polyploid cells. Although endomitosis is biologically important, its regulatory aspects remain elusive. Psychosine, a lysogalactosylceramide, prevents proper cytokinesis when supplemented to proliferating cells. Cytokinetic inhibition by psychosine does not inhibit genome duplication. Consequently cells undergo multiple rounds of endomitotic cell cycles, resulting in the formation of giant multiploid cells. Here we successfully quantified psychosine-triggered multiploid cell formation, showing that membrane sphingolipids ratios modulate psychosine-triggered polyploidy in Namalwa cells. Among enzymes that experimentally remodel cellular sphingolipids, overexpression of glucosylceramide synthase to biosynthesize glycosylsphingolipids (GSLs) and neutral sphingomyelinase 2 to hydrolyze sphingomyelin (SM) additively enhanced psychosine-triggered multiploidy; almost all of the cells became polyploid. In the presence of psychosine, Namalwa cells showed attenuated cell surface SM clustering and suppression of phosphatidylinositol 4,5-bisphosphate production at the cleavage furrow, both important processes for cytokinesis. Depending on the sphingolipid balance between GSLs and SM, Namalwa cells could be effectively converted to viable multiploid cells with psychosine. |
format | Online Article Text |
id | pubmed-4927278 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49272782016-09-16 Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow Watanabe, Hiroshi Okahara, Kyohei Naito-Matsui, Yuko Abe, Mitsuhiro Go, Shinji Inokuchi, Jinichi Okazaki, Toshiro Kobayashi, Toshihide Kozutsumi, Yasunori Oka, Shogo Takematsu, Hiromu Mol Biol Cell Articles Endomitosis is a special type of mitosis in which only cytokinesis—the final step of the cell division cycle—is defective, resulting in polyploid cells. Although endomitosis is biologically important, its regulatory aspects remain elusive. Psychosine, a lysogalactosylceramide, prevents proper cytokinesis when supplemented to proliferating cells. Cytokinetic inhibition by psychosine does not inhibit genome duplication. Consequently cells undergo multiple rounds of endomitotic cell cycles, resulting in the formation of giant multiploid cells. Here we successfully quantified psychosine-triggered multiploid cell formation, showing that membrane sphingolipids ratios modulate psychosine-triggered polyploidy in Namalwa cells. Among enzymes that experimentally remodel cellular sphingolipids, overexpression of glucosylceramide synthase to biosynthesize glycosylsphingolipids (GSLs) and neutral sphingomyelinase 2 to hydrolyze sphingomyelin (SM) additively enhanced psychosine-triggered multiploidy; almost all of the cells became polyploid. In the presence of psychosine, Namalwa cells showed attenuated cell surface SM clustering and suppression of phosphatidylinositol 4,5-bisphosphate production at the cleavage furrow, both important processes for cytokinesis. Depending on the sphingolipid balance between GSLs and SM, Namalwa cells could be effectively converted to viable multiploid cells with psychosine. The American Society for Cell Biology 2016-07-01 /pmc/articles/PMC4927278/ /pubmed/27170180 http://dx.doi.org/10.1091/mbc.E15-08-0555 Text en © 2016 Watanabe et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Watanabe, Hiroshi Okahara, Kyohei Naito-Matsui, Yuko Abe, Mitsuhiro Go, Shinji Inokuchi, Jinichi Okazaki, Toshiro Kobayashi, Toshihide Kozutsumi, Yasunori Oka, Shogo Takematsu, Hiromu Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow |
title | Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow |
title_full | Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow |
title_fullStr | Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow |
title_full_unstemmed | Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow |
title_short | Psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow |
title_sort | psychosine-triggered endomitosis is modulated by membrane sphingolipids through regulation of phosphoinositide 4,5-bisphosphate production at the cleavage furrow |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927278/ https://www.ncbi.nlm.nih.gov/pubmed/27170180 http://dx.doi.org/10.1091/mbc.E15-08-0555 |
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