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Comparative Analysis of Cas9 Activators Across Multiple Species
Several groups have generated programmable transcription factors based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expressi...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2016
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927356/ https://www.ncbi.nlm.nih.gov/pubmed/27214048 http://dx.doi.org/10.1038/nmeth.3871 |
| _version_ | 1782440262616743936 |
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| author | Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Collins, James J Church, George |
| author_facet | Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Collins, James J Church, George |
| author_sort | Chavez, Alejandro |
| collection | PubMed |
| description | Several groups have generated programmable transcription factors based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems and assess the role of cooperativity in maximizing gene expression. |
| format | Online Article Text |
| id | pubmed-4927356 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2016 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-49273562016-11-23 Comparative Analysis of Cas9 Activators Across Multiple Species Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Collins, James J Church, George Nat Methods Article Several groups have generated programmable transcription factors based on the versatile Cas9 protein, yet their relative potency and effectiveness across various cell types and species remain unexplored. Here, we compare Cas9 activator systems and examine their ability to induce robust gene expression in several human, mouse, and fly cell lines. We also explore the potential for improved activation through the combination of the most potent activator systems and assess the role of cooperativity in maximizing gene expression. 2016-05-23 2016-07 /pmc/articles/PMC4927356/ /pubmed/27214048 http://dx.doi.org/10.1038/nmeth.3871 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Chavez, Alejandro Tuttle, Marcelle Pruitt, Benjamin W Ewen-Campen, Ben Chari, Raj Ter-Ovanesyan, Dmitry Haque, Sabina J Cecchi, Ryan J Kowal, Emma J K Buchthal, Joanna Housden, Benjamin E Perrimon, Norbert Collins, James J Church, George Comparative Analysis of Cas9 Activators Across Multiple Species |
| title | Comparative Analysis of Cas9 Activators Across Multiple Species |
| title_full | Comparative Analysis of Cas9 Activators Across Multiple Species |
| title_fullStr | Comparative Analysis of Cas9 Activators Across Multiple Species |
| title_full_unstemmed | Comparative Analysis of Cas9 Activators Across Multiple Species |
| title_short | Comparative Analysis of Cas9 Activators Across Multiple Species |
| title_sort | comparative analysis of cas9 activators across multiple species |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927356/ https://www.ncbi.nlm.nih.gov/pubmed/27214048 http://dx.doi.org/10.1038/nmeth.3871 |
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