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Designing, Packaging, and Delivery of High Titer CRISPR Retro and Lentiviruses via Stereotaxic Injection

Replication defective lentiviruses or retroviruses are capable of stably integrating transgenes into the genome of an infected host cell. This technique has been widely used to encode fluorescent proteins, opto- or chemo-genetic controllers of cell activity, or heterologous expression of human genes...

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Detalles Bibliográficos
Autores principales: Fricano-Kugler, Catherine J., Williams, Michael R., Salinaro, Julia R., Li, Meijie, Luikart, Bryan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927708/
https://www.ncbi.nlm.nih.gov/pubmed/27285851
http://dx.doi.org/10.3791/53783