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Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples

Despite their important role in tissues, fluids and foods, the analysis of non-esterified fatty acids (NEFA) as methyl esters (NEFAME) is performed using expensive, cumbersome and time-consuming procedures that needs of isolation, fractionation and derivatization steps. However, Yi et al. [1] • Incr...

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Autores principales: Pimentel, Lígia L., Fontes, Ana L., Gomes, Ana M., Rodríguez-Alcalá, Luis M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928718/
https://www.ncbi.nlm.nih.gov/pubmed/27408824
http://dx.doi.org/10.1016/j.mex.2015.11.006
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author Pimentel, Lígia L.
Fontes, Ana L.
Gomes, Ana M.
Rodríguez-Alcalá, Luis M.
author_facet Pimentel, Lígia L.
Fontes, Ana L.
Gomes, Ana M.
Rodríguez-Alcalá, Luis M.
author_sort Pimentel, Lígia L.
collection PubMed
description Despite their important role in tissues, fluids and foods, the analysis of non-esterified fatty acids (NEFA) as methyl esters (NEFAME) is performed using expensive, cumbersome and time-consuming procedures that needs of isolation, fractionation and derivatization steps. However, Yi et al. [1] • Increment of the transesterification performance by adding hexane to the reaction mixture, decreasing the time for the derivatization of acylglycerols from 10 min to 3–4 min and stopping the reaction with sulfuric acid. • Avoid cross-contamination of the NEFAME extract by adding 500 μL of water after collection of EFA methyl esters (EFAME). • Samples are spiked with three internal standards: a triacylglycerol (to calculate the concentration of EFA), a free fatty acid (to calculate NEFA) and a FAME (to control isolation of FAME and cross-contamination).
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spelling pubmed-49287182016-07-12 Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples Pimentel, Lígia L. Fontes, Ana L. Gomes, Ana M. Rodríguez-Alcalá, Luis M. MethodsX Agricultural and Biological Science Despite their important role in tissues, fluids and foods, the analysis of non-esterified fatty acids (NEFA) as methyl esters (NEFAME) is performed using expensive, cumbersome and time-consuming procedures that needs of isolation, fractionation and derivatization steps. However, Yi et al. [1] • Increment of the transesterification performance by adding hexane to the reaction mixture, decreasing the time for the derivatization of acylglycerols from 10 min to 3–4 min and stopping the reaction with sulfuric acid. • Avoid cross-contamination of the NEFAME extract by adding 500 μL of water after collection of EFA methyl esters (EFAME). • Samples are spiked with three internal standards: a triacylglycerol (to calculate the concentration of EFA), a free fatty acid (to calculate NEFA) and a FAME (to control isolation of FAME and cross-contamination). Elsevier 2015-11-23 /pmc/articles/PMC4928718/ /pubmed/27408824 http://dx.doi.org/10.1016/j.mex.2015.11.006 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Agricultural and Biological Science
Pimentel, Lígia L.
Fontes, Ana L.
Gomes, Ana M.
Rodríguez-Alcalá, Luis M.
Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples
title Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples
title_full Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples
title_fullStr Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples
title_full_unstemmed Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples
title_short Considerations about the in situ derivatization and fractionation of EFA and NEFA in biological and food samples
title_sort considerations about the in situ derivatization and fractionation of efa and nefa in biological and food samples
topic Agricultural and Biological Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4928718/
https://www.ncbi.nlm.nih.gov/pubmed/27408824
http://dx.doi.org/10.1016/j.mex.2015.11.006
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