Cargando…

RNA Polymerase II cluster dynamics predict mRNA output in living cells

Protein clustering is a hallmark of genome regulation in mammalian cells. However, the dynamic molecular processes involved make it difficult to correlate clustering with functional consequences in vivo. We developed a live-cell super-resolution approach to uncover the correlation between mRNA synth...

Descripción completa

Detalles Bibliográficos
Autores principales:	Cho, Won-Ki, Jayanth, Namrata, English, Brian P, Inoue, Takuma, Andrews, J Owen, Conway, William, Grimm, Jonathan B, Spille, Jan-Hendrik, Lavis, Luke D, Lionnet, Timothée, Cisse, Ibrahim I
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	eLife Sciences Publications, Ltd 2016
Materias:	Biophysics and Structural Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4929003/ https://www.ncbi.nlm.nih.gov/pubmed/27138339 http://dx.doi.org/10.7554/eLife.13617

Ejemplares similares

Mapping translation 'hot-spots' in live cells by tracking single molecules of mRNA and ribosomes
por: Katz, Zachary B, et al.
Publicado: (2016)

Single-molecule tracking of the transcription cycle by sub-second RNA detection
por: Zhang, Zhengjian, et al.
Publicado: (2014)

Structural mechanism of ATP-independent transcription initiation by RNA polymerase I
por: Han, Yan, et al.
Publicado: (2017)

Complete dissection of transcription elongation reveals slow translocation of RNA polymerase II in a linear ratchet mechanism
por: Dangkulwanich, Manchuta, et al.
Publicado: (2013)

3D imaging of Sox2 enhancer clusters in embryonic stem cells
por: Liu, Zhe, et al.
Publicado: (2014)

qSR: a quantitative super-resolution analysis tool reveals the cell-cycle dependent organization of RNA Polymerase I in live human cells
por: Andrews, J. O., et al.
Publicado: (2018)

Real-time imaging of Huntingtin aggregates diverting target search and gene transcription
por: Li, Li, et al.
Publicado: (2016)

Robust model-based analysis of single-particle tracking experiments with Spot-On
por: Hansen, Anders S, et al.
Publicado: (2018)

A tethered delivery mechanism explains the catalytic action of a microtubule polymerase
por: Ayaz, Pelin, et al.
Publicado: (2014)

Live-cell single-molecule tracking reveals co-recognition of H3K27me3 and DNA targets polycomb Cbx7-PRC1 to chromatin
por: Zhen, Chao Yu, et al.
Publicado: (2016)

Fidaxomicin jams Mycobacterium tuberculosis RNA polymerase motions needed for initiation via RbpA contacts
por: Boyaci, Hande, et al.
Publicado: (2018)

Mechanism for priming DNA synthesis by yeast DNA Polymerase α
por: Perera, Rajika L, et al.
Publicado: (2013)

Single-molecule visualization of fast polymerase turnover in the bacterial replisome
por: Lewis, Jacob S, et al.
Publicado: (2017)

The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA
por: Soundararajan, Harish Chandra, et al.
Publicado: (2014)

A novel SH2 recognition mechanism recruits Spt6 to the doubly phosphorylated RNA polymerase II linker at sites of transcription
por: Sdano, Matthew A, et al.
Publicado: (2017)

Kinetic competition during the transcription cycle results in stochastic RNA processing
por: Coulon, Antoine, et al.
Publicado: (2014)

Recognition of the small regulatory RNA RydC by the bacterial Hfq protein
por: Dimastrogiovanni, Daniela, et al.
Publicado: (2014)

Dissection of specific binding of HIV-1 Gag to the 'packaging signal' in viral RNA
por: Comas-Garcia, Mauricio, et al.
Publicado: (2017)

Reconstitution of selective HIV-1 RNA packaging in vitro by membrane-bound Gag assemblies
por: Carlson, Lars-Anders, et al.
Publicado: (2016)

cryo-EM structures of the E. coli replicative DNA polymerase reveal its dynamic interactions with the DNA sliding clamp, exonuclease and τ
por: Fernandez-Leiro, Rafael, et al.
Publicado: (2015)

The export receptor Crm1 forms a dimer to promote nuclear export of HIV RNA
por: Booth, David S, et al.
Publicado: (2014)

Drosophila Nanos acts as a molecular clamp that modulates the RNA-binding and repression activities of Pumilio
por: Weidmann, Chase A, et al.
Publicado: (2016)

Recruitment of two dyneins to an mRNA-dependent Bicaudal D transport complex
por: Sladewski, Thomas E, et al.
Publicado: (2018)

Nuclear microenvironments modulate transcription from low-affinity enhancers
por: Tsai, Albert, et al.
Publicado: (2017)

Single-molecule tracking in live cells reveals distinct target-search strategies of transcription factors in the nucleus
por: Izeddin, Ignacio, et al.
Publicado: (2014)

Structure of an inactive RNA polymerase II dimer
por: Aibara, Shintaro, et al.
Publicado: (2021)

Single-molecule view of coordination in a multi-functional DNA polymerase
por: Pauszek, Raymond F, et al.
Publicado: (2021)

Transcribing RNA polymerase III observed by electron cryomicroscopy
por: Hoffmann, Niklas A., et al.
Publicado: (2016)

Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction
por: Ranjan, Anand, et al.
Publicado: (2020)

May I check your cap?
por: Geyer, Elisabeth A, et al.
Publicado: (2016)

Structural explanation for the role of Mn(2+) in the activity of ϕ6 RNA-dependent RNA polymerase
por: Poranen, Minna M., et al.
Publicado: (2008)

Allosteric communication in DNA polymerase clamp loaders relies on a critical hydrogen-bonded junction
por: Subramanian, Subu, et al.
Publicado: (2021)

Structural and functional analyses of the interaction of archaeal RNA polymerase with DNA
por: Wojtas, Magdalena N., et al.
Publicado: (2012)

Structure of the bacteriophage PhiKZ non-virion RNA polymerase
por: de Martín Garrido, Natàlia, et al.
Publicado: (2021)

A complex IRES at the 5'-UTR of a viral mRNA assembles a functional 48S complex via an uAUG intermediate
por: Neupane, Ritam, et al.
Publicado: (2020)

Regulation of RUVBL1-RUVBL2 AAA-ATPases by the nonsense-mediated mRNA decay factor DHX34, as evidenced by Cryo-EM
por: López-Perrote, Andres, et al.
Publicado: (2020)

Acidic C-terminal domains autoregulate the RNA chaperone Hfq
por: Santiago-Frangos, Andrew, et al.
Publicado: (2017)

Cryo-EM structures reveal high-resolution mechanism of a DNA polymerase sliding clamp loader
por: Gaubitz, Christl, et al.
Publicado: (2022)

Motility and microtubule depolymerization mechanisms of the Kinesin-8 motor, KIF19A
por: Wang, Doudou, et al.
Publicado: (2016)

Correction: Sequential conformational rearrangements in flavivirus membrane fusion
por: Chao, Luke H, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_aqua6n0rkr6sb8g1g7se1hufo8