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Measurement of Rapid Amiloride-Dependent pH Changes at the Cell Surface Using a Proton-Sensitive Field-Effect Transistor

We present a novel method for the rapid measurement of pH fluxes at close proximity to the surface of the plasma membrane in mammalian cells using an ion-sensitive field-effect transistor (ISFET). In conjuction with an efficient continuous superfusion system, the ISFET sensor was capable of recordin...

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Detalles Bibliográficos
Autores principales: Schaffhauser, Daniel, Fine, Michael, Tabata, Miyuki, Goda, Tatsuro, Miyahara, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4931471/
https://www.ncbi.nlm.nih.gov/pubmed/27043644
http://dx.doi.org/10.3390/bios6020011