Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR

BACKGROUND: After transformation, plants that are homozygous and contain one copy of the transgene are typically selected for further study. If real-time PCR is to be used to determine copy number and zygosity, it must be able to distinguish hemizygous from homozygous and one-copy from two-copy plan...

Descripción completa

Detalles Bibliográficos
Autores principales: Bubner, Ben, Gase, Klaus, Baldwin, Ian T
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC493272/
https://www.ncbi.nlm.nih.gov/pubmed/15251044
http://dx.doi.org/10.1186/1472-6750-4-14

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC493272/
https://www.ncbi.nlm.nih.gov/pubmed/15251044
http://dx.doi.org/10.1186/1472-6750-4-14

Ejemplares similares