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Two-fold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR

BACKGROUND: After transformation, plants that are homozygous and contain one copy of the transgene are typically selected for further study. If real-time PCR is to be used to determine copy number and zygosity, it must be able to distinguish hemizygous from homozygous and one-copy from two-copy plan...

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Detalles Bibliográficos
Autores principales:	Bubner, Ben, Gase, Klaus, Baldwin, Ian T
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2004
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC493272/ https://www.ncbi.nlm.nih.gov/pubmed/15251044 http://dx.doi.org/10.1186/1472-6750-4-14

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