Destabilizing an interacting motif strengthens the association of a designed ankyrin repeat protein with tubulin

Affinity maturation by random mutagenesis and selection is an established technique to make binding molecules more suitable for applications in biomedical research, diagnostics and therapy. Here we identified an unexpected novel mechanism of affinity increase upon in vitro evolution of a tubulin-spe...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahmad, Shoeb, Pecqueur, Ludovic, Dreier, Birgit, Hamdane, Djemel, Aumont-Nicaise, Magali, Plückthun, Andreas, Knossow, Marcel, Gigant, Benoît
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933879/
https://www.ncbi.nlm.nih.gov/pubmed/27380724
http://dx.doi.org/10.1038/srep28922

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933879/
https://www.ncbi.nlm.nih.gov/pubmed/27380724
http://dx.doi.org/10.1038/srep28922

Ejemplares similares