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“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images

In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-lim...

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Detalles Bibliográficos
Autores principales: Mortensen, Kim I., Flyvbjerg, Henrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933971/
https://www.ncbi.nlm.nih.gov/pubmed/27381055
http://dx.doi.org/10.1038/srep28680