“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images

In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-lim...

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Detalles Bibliográficos
Autores principales: Mortensen, Kim I., Flyvbjerg, Henrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933971/
https://www.ncbi.nlm.nih.gov/pubmed/27381055
http://dx.doi.org/10.1038/srep28680
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author Mortensen, Kim I.
Flyvbjerg, Henrik
author_facet Mortensen, Kim I.
Flyvbjerg, Henrik
author_sort Mortensen, Kim I.
collection PubMed
description In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-limited spots in any image taken by the camera, thus making dedicated calibration procedures redundant by enabling calibration post festum, from images filed without calibration information.
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spelling pubmed-49339712016-07-08 “Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images Mortensen, Kim I. Flyvbjerg, Henrik Sci Rep Article In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-limited spots in any image taken by the camera, thus making dedicated calibration procedures redundant by enabling calibration post festum, from images filed without calibration information. Nature Publishing Group 2016-07-06 /pmc/articles/PMC4933971/ /pubmed/27381055 http://dx.doi.org/10.1038/srep28680 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mortensen, Kim I.
Flyvbjerg, Henrik
“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
title “Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
title_full “Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
title_fullStr “Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
title_full_unstemmed “Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
title_short “Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
title_sort “calibration-on-the-spot”: how to calibrate an emccd camera from its images
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933971/
https://www.ncbi.nlm.nih.gov/pubmed/27381055
http://dx.doi.org/10.1038/srep28680
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