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“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-lim...
Autores principales: | Mortensen, Kim I., Flyvbjerg, Henrik |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4933971/ https://www.ncbi.nlm.nih.gov/pubmed/27381055 http://dx.doi.org/10.1038/srep28680 |
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