Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification

Dumbbell probe (DP) attracts increasing interests in rolling circle amplification (RCA). A universal DP production method through cleavage-ligation of hairpin was proposed and optimized. The production is characterized by restriction endonuclease (RE)-induced cleavage ends ligation. It has the advan...

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Detalles Bibliográficos
Autores principales: Wei, Hua, Tang, Suming, Hu, Tianyu, Zhao, Guojie, Guan, Yifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935871/
https://www.ncbi.nlm.nih.gov/pubmed/27385060
http://dx.doi.org/10.1038/srep29229

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4935871/
https://www.ncbi.nlm.nih.gov/pubmed/27385060
http://dx.doi.org/10.1038/srep29229

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