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DNA End Resection: Facts and Mechanisms
DNA double-strand breaks (DSBs), which arise following exposure to a number of endogenous and exogenous agents, can be repaired by either the homologous recombination (HR) or non-homologous end-joining (NHEJ) pathways in eukaryotic cells. A vital step in HR repair is DNA end resection, which generat...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4936662/ https://www.ncbi.nlm.nih.gov/pubmed/27240470 http://dx.doi.org/10.1016/j.gpb.2016.05.002 |
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author | Liu, Ting Huang, Jun |
author_facet | Liu, Ting Huang, Jun |
author_sort | Liu, Ting |
collection | PubMed |
description | DNA double-strand breaks (DSBs), which arise following exposure to a number of endogenous and exogenous agents, can be repaired by either the homologous recombination (HR) or non-homologous end-joining (NHEJ) pathways in eukaryotic cells. A vital step in HR repair is DNA end resection, which generates a long 3′ single-stranded DNA (ssDNA) tail that can invade the homologous DNA strand. The generation of 3′ ssDNA is not only essential for HR repair, but also promotes activation of the ataxia telangiectasia and Rad3-related protein (ATR). Multiple factors, including the MRN/X complex, C-terminal-binding protein interacting protein (CtIP)/Sae2, exonuclease 1 (EXO1), Bloom syndrome protein (BLM)/Sgs1, DNA2 nuclease/helicase, and several chromatin remodelers, cooperate to complete the process of end resection. Here we review the basic machinery involved in DNA end resection in eukaryotic cells. |
format | Online Article Text |
id | pubmed-4936662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-49366622016-07-14 DNA End Resection: Facts and Mechanisms Liu, Ting Huang, Jun Genomics Proteomics Bioinformatics Review DNA double-strand breaks (DSBs), which arise following exposure to a number of endogenous and exogenous agents, can be repaired by either the homologous recombination (HR) or non-homologous end-joining (NHEJ) pathways in eukaryotic cells. A vital step in HR repair is DNA end resection, which generates a long 3′ single-stranded DNA (ssDNA) tail that can invade the homologous DNA strand. The generation of 3′ ssDNA is not only essential for HR repair, but also promotes activation of the ataxia telangiectasia and Rad3-related protein (ATR). Multiple factors, including the MRN/X complex, C-terminal-binding protein interacting protein (CtIP)/Sae2, exonuclease 1 (EXO1), Bloom syndrome protein (BLM)/Sgs1, DNA2 nuclease/helicase, and several chromatin remodelers, cooperate to complete the process of end resection. Here we review the basic machinery involved in DNA end resection in eukaryotic cells. Elsevier 2016-06 2016-05-27 /pmc/articles/PMC4936662/ /pubmed/27240470 http://dx.doi.org/10.1016/j.gpb.2016.05.002 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Liu, Ting Huang, Jun DNA End Resection: Facts and Mechanisms |
title | DNA End Resection: Facts and Mechanisms |
title_full | DNA End Resection: Facts and Mechanisms |
title_fullStr | DNA End Resection: Facts and Mechanisms |
title_full_unstemmed | DNA End Resection: Facts and Mechanisms |
title_short | DNA End Resection: Facts and Mechanisms |
title_sort | dna end resection: facts and mechanisms |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4936662/ https://www.ncbi.nlm.nih.gov/pubmed/27240470 http://dx.doi.org/10.1016/j.gpb.2016.05.002 |
work_keys_str_mv | AT liuting dnaendresectionfactsandmechanisms AT huangjun dnaendresectionfactsandmechanisms |